Fig. 5: Chromatin remodeling subunits bind GRS site near INO1 locus.

a BirA*-dCas9 approach designed to target protospacer adjacent motif (PAM) sites in the vicinity of GRS I site by using different sgRNAs for site-specific binding of BirA*-dCas9 and biotinylation of proximal proteins. Proteins were then affinity purified with streptavidin, digested, and analyzed by mass spectrometry. b, d Micro array (MA) plots representing average protein intensities from biological triplicates from sgRNA-BirA*-dCas9 samples S5 (b) and S7 (c) plotted against protein quantity fold changes from these sgRNA-BirA*-dCas9 samples over those from their respective negative control triplicates (BirA*-dCas9). Protein fold change p-values calculated from a two-sided unpaired Student’s t test with equal variance are color coded, and the gray dashed lines represent 1.5-fold quantity increase. p-values are indicated next to labels. c, e Box plots of measured protein intensities of candidates from sgRNA-BirA*-dCas9 samples S5 (d) and S7 (e) triplicates along with their respective negative control triplicates. Source data are provided as a Source Data file. Median is indicated as middle line, average as a white dot, 25th and 75th percentile as boxes and whiskers represent 5th and 95th percentile. False discovery rates thresholds for mass spectrometry identification of peptide spectrum match, peptides, and proteins were set to 0.1. See also Supplementary Data 1–6 and Supplementary Figs. 5 and 6.