Fig. 6: Interactions between MTREC and CPF. | Nature Communications

Fig. 6: Interactions between MTREC and CPF.

From: Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex

Fig. 6: Interactions between MTREC and CPF.The alternative text for this image may have been generated using AI.

A ITC titration of Iss1 into Pla1RRM. B ITC titration of Red1288-345 into a pre-formed complex of Pla1RRM-Iss130-76. C ITC titration of Iss1 into a pre-formed complex of Pla1RRM-Red1288-345. The calculated dissociation constants (KD) from an average of two independent measurements are shown. BLI kinetic analyses of biotinylated Red1288-345 and Iss130-76 with varying concentrations of Pla1FL are shown in panels D and E, respectively. Black lines represent fits to the experimental data obtained using a 1:1 binding global fitting model. F Y2H experiments show interaction between Msi2 fused to G4BD and MTREC components Mmi1 or Pab2 fused to G4AD compared to an auto-activation control (ctrl).

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