Fig. 5: Phosphatidylcholine binding splenic B1a cells are reduced in EVH1−/− mice. | Nature Communications

Fig. 5: Phosphatidylcholine binding splenic B1a cells are reduced in EVH1−/− mice.

From: An Igh distal enhancer modulates antigen receptor diversity by determining locus conformation

Fig. 5: Phosphatidylcholine binding splenic B1a cells are reduced in EVH1−/− mice.The alternative text for this image may have been generated using AI.

a Representative flow cytometry plots of splenocytes gated for B1 B cells were assessed using FVS-510 and antibodies (CD19-PerCp, B220-APC Cy7, CD23-FITC, IgM-e450, IgD-AF700). b, d Frequencies of B1 B cells were averaged and each symbol represents results from one mouse. b Average frequency of B1 B cells (CD19+B220loCD23-IgMhiIgDlo) was assessed in three independent experiments. WT (n = 35), EVH1−/− (n = 19) mice. P values from unpaired two-tailed Student’s t test. c Representative flow cytometry plots of splenocytes gated for B1 B cells using FVS-510 and antibodies (CD19-PerCp, B220-APC Cy7, CD43-APC, IgM-e450, IgD AF700) and PtC liposome (FITC). d Average frequency of B1 B cells binding PtC (CD19+B220loCD43+IgMhiIgDloPtC+). WT (n = 5), EVH1−/− (n = 5) mice. P values from unpaired two-tailed Student’s t test.

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