Fig. 1: NMR-observed conformational states of A2AAR-ligand complexes compared in two different membrane mimetics. | Nature Communications

Fig. 1: NMR-observed conformational states of A2AAR-ligand complexes compared in two different membrane mimetics.

From: Anionic phospholipids control mechanisms of GPCR-G protein recognition

Fig. 1

a The 1-dimensional 19F-NMR spectra of A2AAR[A289CTET] reconstituted into DDM/CHS mixed micelles and in complexes with the antagonist ZM241385 and agonist NECA. On the right, the NMR spectra shown on the left are interpreted by Lorenztian deconvolutions with the minimal number of components that provided a good fit, labeled P1 to P4. The chemical shifts of P1 to P4 are indicated by the colored dashed vertical lines. b The 1-dimensional 19F-NMR spectra of A2AAR[A289CTET] reconstituted into lipid nanodiscs containing the lipids POPC and POPS in a 70:30 molar ratio and in complexes with the same antagonist and agonist. Same presentation details as in a. c Relative populations of each state represented in a bar chart format. Source data are included as a Source Data file.

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