Fig. 6: Visualization of the role of anionic lipids in complex formation of A2AAR with GαS.
From: Anionic phospholipids control mechanisms of GPCR-G protein recognition
a, b Schematic side views of a apo and b antagonist-bound A2AAR in phospholipid membranes without and with anionic lipids, colored blue and orange, respectively. c, d Schematic view of agonist-bound A2AAR and mini-GαS in membranes containing c only zwitterionic phospholipids and d zwitterionic and anionic phospholipids. The black arrows signify an equilibrium between mini-GαS bound A2AAR and A2AAR bound to agonist alone, and the length of the arrows signifies how anionic lipids shift this equilibrium toward formation of the ternary complex. This figure was prepared with Biorender.com.
