Fig. 1: Antagonistic and biocontrol activity of P. mosselii 923.
a, b Antagonistic activity of strain 923 towards Xoo PXO99A, Xoc RS105, and M. oryzae isolate R01-1 in co-cultured assays, untreated strain 923 was used as control (CK). c Xoc RS105-Gus lesion lengths on Yuanfengzao rice inoculated with strain 923, ΔpsdA or 923 SUP at 7 d post-inoculation (dpi) in the greenhouse. ‘Pre’ represents rice leaves pretreated with water (MOCK-Pre), 923, ΔpsdA or 923 SUP (supernatant) prior to pathogen inoculation; ‘Tre’ represents rice leaves inoculated with Xoc RS105-Gus and then treated with water (MOCK-Tre), 923, ΔpsdA or 923 SUP. d Population dynamics of Xoc RS105-Gus in rice leaves. Bacterial populations were monitored by the GUS quantification and histochemical staining at 7 dpi. Error bars show means ± SD (n = 3 independent leaves) and significant differences at ***P < 0.001, ***P = 6.4 × 10−14, = 3.2 × 10−15, = 8.3 × 10−15, = 5.5 × 10−16 in sequence of c, ***P < 0.001, ***P = 1.3 × 10−25, = 2.4 × 10−26, = 1.8 × 10-25, = 3.6 × 10-26 in sequence of d, ns not significant (P > 0.05). e, f Disease lesions areas on field-grown rice inoculated with strain 923 and (e) Xoo PXO99A or (f) Xoc RS105. Lesion areas were determined at 15 dpi (n = 15 independent leaves, means ± SD), ***P < 0.001, ***P = 1.2×10−15, = 1.3×10−14 in sequence of e; ***P < 0.001, ***P = 4.7 × 10−13, = 3.2 × 10−11 in sequence of f; ns, not significant (P > 0.05). The statistical significance of the lesion areas inoculated with Xoc or Xoo was determined using the LSD test method and one-way ANOVA; The center bar represents the mean, and Min to Max of box and whiskers was used. The experiments were repeated three times independently with similar results.
