Fig. 7: Effect of contractility and differentiation perturbation on the curvature-induced organization.
From: Emergent collective organization of bone cells in complex curvature fields
a Representative maximum intensity projections (F-actin) on the convex unduloids (top) and catenoids (bottom), displaying the effects of contractility inhibition (Bleb.), contractility enhancement (TGF-β), and differentiation inhibition (-AA/BGP). b Intensity reduction as a function of \({\delta }_{{\kappa }_{2} < 0}\) for the convex spheres. The top chart demonstrates the effects of contractility perturbation (Kruskal–Wallis test: ****p < 0.0001). The bottom chart demonstrates the effects of differentiation inhibition (Two-tailed Mann–Whitney test: **p = 0.0057, ****p < 0.0001). The solid lines represent the median values while the shaded areas represent the interquartile range. c Effect of contractility perturbation and differentiation inhibition on cell sheet detachment over concave hemispheres at day 8. d Quantification of cell sheet displacement (Δh) due to contractility perturbation or differentiation inhibition. Data from multiple regions on three independent experiments. Data are presented as mean values +/− SD. Brown-Forsythe and Welch’s ANOVA with Dunnett’s T3 multiple comparisons: **p = 0.0017, ****p < 0.0001. e Merged F-actin (red) and DNA (blue) expression at day 8 in single slices from z-stacks obtained on concave spherical wells, showing differences in SF morphology and orientation for the different culture conditions. f PD of the SF orientation on maximum intensity projections of concave spheres. Data from three independent experiments. The red lines represent Epanechnikov kernel density estimates. All scale bars are 50 μm. Source data are provided as a Source Data file.
