Fig. 1: Proteomic analysis of enriched osteoclast secretory lysosomes identifies Slc37a2. | Nature Communications

Fig. 1: Proteomic analysis of enriched osteoclast secretory lysosomes identifies Slc37a2.

From: Sugar transporter Slc37a2 regulates bone metabolism in mice via a tubular lysosomal network in osteoclasts

Fig. 1: Proteomic analysis of enriched osteoclast secretory lysosomes identifies Slc37a2.

a Experimental setup for SPION ‘pulse-chase’ and representative confocal images of mouse bone marrow monocyte (BMMs) differentiation into mature αvβ3-integrin-positive (IntegriSense645) osteoclasts following RANKL stimulation. Bar 150 µm. b Quantitation of IntegriSense645 positive cells during RANKL stimulation (n = 3 biologically independent cells from 2 experiments). Data are presented as mean percentage (%) ± SD. c Schema of the SPION-based SL enrichment method, validation, and proteomic analysis. Created with BioRender.com. d Immunoblotting for protein markers of various subcellular compartments in whole-cell homogenates (H), post-nuclear supernatant (PNS), magnetic column flow-through (UB), and enriched SL fractions (F1–F3) (n = 3). LRO lysosome-related organelle. e Volcano plot depicting the difference between proteins in whole cell homogenates and secretory lysosomes (SLs) (n = 3, P > 0.05, ANOVA, Benjamini–Hochberg adjusted). The top 218 proteins up-regulated in the lysosomal fractions are shown in green (FC > 1.5, P < 0.05) with the top 6 membrane transporters colored in purple and Slc37a2 highlighted in red.

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