Fig. 5: Enhancer self-transcription decreases the local concentration of the Dl activator.
From: Dynamic interplay between non-coding enhancer transcription and gene activity in development
a Working hypothesis; elongating Pol II mediates eviction of transcription factors from the enhancer region. b Representative Airyscan images of Dorsal-GFP and PP7 transcription dots in the single z-plane containing the brightest PP7 signal. The white square indicates the analyzed region (29 × 29 pixels) centering the brightest PP7 signal. Scale bar indicates 3 μm. c Heatmaps showing the averaged distribution of Dorsal-GFP centering the PP7 transcription site or random site. A total of 839 and 852 PP7-transcribing nuclei, respectively, were obtained from 50 independent embryos for the reporter locus driving PP7 transcription in an outward (left) or an inward orientation (right). d Radial profiles of the averaged Dorsal-GFP distribution are shown in (c). Error bar represents the standard error of the mean. The difference between the areas of distribution curves of the transcription site was tested by a two-sided Wilcoxon rank-sum test (p = 0.02). Source data are provided as a Source Data file.
