Fig. 6: Genetic complementation experiments using Psyn thyX.
From: Bacterial origins of thymidylate metabolism in Asgard archaea and Eukarya
a Map for the synthetic plasmid pTwist-Psyn-ThyX. Transcription of the Psyn thyX gene is under the control of a synthetic T5 promoter carrying lacO. An ampicillin resistance marker and a p15A replication origin are integrated, as well as a lambda t0 terminator. b Interdomain complementation assay using Psyn thyX. The ability of Psyn thyX to permit thymidine-independent growth of E. coli thymidine-auxotroph FE01311 (ΔthyA, lacI) was checked after three days at 37 °C in the presence of 1 mM IPTG using minimal M9 medium. 1 and 2 correspond to two independent clones of Psyn thyX/FE013 whereas 3 and 4 are negative (no insert) and positive (C. trachomatics thyX) controls, respectively. The plate on the left contains IPTG, but no thymidine, whereas the plate on the right contains thymidine, but no IPTG.
