Table 2 Kinetic parameters of LsAAE3 in the presence or absence of LsBOS

From: Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus

Reaction

Oxalate

L-DAP

CoA

kcat (s−1)

Km (mM)

kcat/Km (s−1M−1)

Km (mM)

kcat/Km (s−1M−1)

Km (mM)

kcat/Km (s−1M−1)

LsAAE3 (pH 6.0)

Oxalate → oxalyl CoA

2.0 ± 0.2

7.5 × 10−2

n/a

n/a

5.0 ± 1.0

3.0 × 10−2

(1.50 ± 0.09) × 10−4

LsAAE3 (pH 8.0)

L-DAP → β-L-ODAP

0.6 ± 0.4

1.9 × 103

2.5 ± 0.5

456

(1.8 ± 0.8) × 10−6

6.3 × 108

1.14 ± 0.10

LsAAE3/LsBOS (pH 8.0)

L-DAP → β-L-ODAP

0.25 ± 0.05

1.2 × 103

0.6 ± 0.1

1.97 × 104

(7.0 ± 1.0) × 10−5

1.69 × 108

11.8 ± 0.76

  1. Presented as mean (n = 3) ± standard deviation.
Back to article page