Table 3 Effect of non-reactive CoA-analogues on LsAAE3 oxalyl-CoA formation and LsAAE3 + LsBOS β-L-ODAP formation

From: Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus

Treatment

Oxalyl-CoA formation (LsAAE3)

β-L-ODAP formation

(LsAAE3 + LsBOS)

 

µmol·s−1·mol

LsAAE3−1

% of product with CoA

µmol·s−1·mol

LsAAE3−1

% of product with CoA

CoA

(1.84 ± 0.11) × 105

100 %

(5.17 ± 0.11) × 106

100 %

Desulpho-CoA

13.58 ± 2.47

0.007 %

(1.17 ± 0.04) × 105

2.3 %

S-ethyl-CoA

10.24 ± 3.73

0.005 %

(1.96 ± 0.09) × 105

3.8%

  1. Oxalate, Mg2+, L-DAP and ATP were present in all reactions. Presented as mean (n = 3) ± standard deviation.
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