Fig. 2: SLIT2 is critical for coadaptation.
a, b Livers from intrasplenic injection mouse models induced with Kpc1199 (a) or Panc02 (b) cells were subjected to transcriptional analysis after confirmation of premetastatic niche (PMN) markers (e.g., CD163, etc.) (n = 3 mice per group, 5 fields assessed per sample). Scale bars, 500 μm for H&E staining or 100 μm for IHC-P; * represents metastatic niches. c Gene set enrichment analysis (GSEA) based on the gene expression profiles of liver tissues undergoing different metastatic processes after mouse modelling with Panc02 or Kpc1199 cells (false discovery rate analysis; NES, normalized enrichment score). d Venn diagram displaying secreted axon guidance genes upregulated in both PMNs and macrometastatic niche (MMN) compared to levels in normal liver (NL) Panc02 and Kpc1199 cells based on the gene expression profiles of liver tissues from mouse models. e Real-time PCR showing the relative mRNA level of SLIT2 in the left lobule of livers from Kpc1199 cell-injected mouse models on D3, D7, D10 or D15 (n = 5 per group, mean ± SEM.; two-tailed unpaired t test). (f) Representative IHC-P staining of H&E, GFP or SLIT2 in the left lobule of livers from Kpc1199 cell-injected mouse models on D3 (NL), D8 (PMN), D15 or D20 (MMN) (n = 5 samples per group, 3 fields assessed per sample); * represents metastatic niches. Scale bars, 100 μm. g, h Representative H&E or IHC-P staining of SLIT2 in PMNs (g) or MMNs (h) formed in the left lobule of livers from KrasG12D/+/Trp53R172H/+/Pdx1-Cre (KPC) mice bearing spontaneous PDAC liver metastasis (n = 6 samples per group, 3 fields assessed per sample). Scale bars, 100 μm. Source data are provided in the Source Data file.
