Fig. 3: Reduced evoked ACh release in DLS in male zQ175+/− mice. | Nature Communications

Fig. 3: Reduced evoked ACh release in DLS in male zQ175+/− mice.

From: Cholinergic deficits selectively boost cortical intratelencephalic control of striatum in male Huntington’s disease model mice

Fig. 3: Reduced evoked ACh release in DLS in male zQ175+/− mice.The alternative text for this image may have been generated using AI.

a Diagram showing AAV-AChSnFr (Acetylcholine sniffer) stereotaxically injected in dorsolateral striatum (DLS). The imaging area and bipolar electrical stimulator are indicated. b DLS section showing the striatal neuropil expressing AAV-AChSnFr and the imaging area (Scale bar: 50 μm). c Representative traces showing a decrease in evoked acetylcholine (ACh) released in zQ175+/− compared to zQ175−/− controls. Scale bar: 0.05 Normalized Fluorescence (Fn). Electrical stimulation (step) consisted of 20 − 1 ms stimuli, of 1 mA delivered at 2 Hz. d Boxplot summarizing the data from zQ175−/− (N = 3; n = 6 slices), zQ175+/− (N = 4; n = 7), **p = 0.0082 Mann–Whitney non-parametric two-sided test. Shown is the % change compared to median data collected in zQ175−/− controls, and the Dynamic response [(Fn – F0) * s], see “Methods”.

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