Fig. 4: Muscarinic activation modulates IT corticostriatal transmission, but not PT. | Nature Communications

Fig. 4: Muscarinic activation modulates IT corticostriatal transmission, but not PT.

From: Cholinergic deficits selectively boost cortical intratelencephalic control of striatum in male Huntington’s disease model mice

Fig. 4: Muscarinic activation modulates IT corticostriatal transmission, but not PT.The alternative text for this image may have been generated using AI.

a Schematic of AAV9.EF1a.DIO.ChR2-GFP delivered in primary motor cortex (M1) of D2-eGFP crossed with PT-cre (Pyramidal tract-cre) male mice. Whole-cell recordings were obtained in DLS from ipsilateral SPNs. b Representative optically evoked EPSCs (oEPSC) traces from direct pathway SPNs (dSPNs, red) and indirect pathway SPNs (iSPNs, black) obtained by stimulating PT terminals. Traces acquired at baseline (control), and during Oxotremorine-M (Oxo-M, 10 μM) perfusion (Scale bars: 10 ms and 50 pA). c Time-series of oEPSC peak amplitudes normalized to control and plotted over time. No effect of five minutes application of Oxo-M (10 μM) on oEPSC amplitude. d Schematics of AAV9.hSyn.Chronos-GFP delivered in M1 of D1-tdTomato or D2-eGFP mice. Whole-cell recordings were obtained from SPNs in contralateral DLS. e Representative oEPSC traces obtained by stimulating intratelencephalic (IT) terminals and recorded from dSPNs (red) and iSPNs (black) before, during, and after (wash) Oxo-M (10 μM) perfusion (Scale bars: 50 ms and 50 pA). f Five minutes Oxo-M application induces a similar and long-lasting reduction of oEPSC amplitude in both dSPNs and iSPNs. g Significant increase in paired pulse ratio (PPR) ratio during Oxo-M perfusion and during the wash period. In dSPNs, control vs. Oxo-M (N = 4; n = 13), **p = 0.0650; control vs. wash (N = 4; n = 13), ****p < 0.0001. In iSPNs, control vs. Oxo-M (N = 4; n = 13), **p = 0.0019; control vs. wash (N = 3; n = 8), **p = 0.0090 in a Kruskal-Wallis test with Dunn’s multiple comparisons. Boxplots represent median and interquartile range, whiskers min/max value. In c and f, shading represent the interquartile range and line the median. All mice used were male.

Back to article page