Fig. 5: OPN knockout inhibits vascular tumour cell colony formation, tumorigenicity and Stat3 phosphorylation. | Nature Communications

Fig. 5: OPN knockout inhibits vascular tumour cell colony formation, tumorigenicity and Stat3 phosphorylation.

From: Autophagy inhibition prevents lymphatic malformation progression to lymphangiosarcoma by decreasing osteopontin and Stat3 signaling

Fig. 5

a Lysates from Ctrl and two clones of OPN KO tumour cells were examined by western blots for OPN and Vinculin. b, c Representative images of colony formation assay of Ctrl and OPN KO tumour cells are shown in b, and mean ± SD of the colony number are shown in c. n = 3 biologically independent cells. d Ctrl and OPN KO tumour cells were subcutaneously injected in recipient nude mice. Mean ± SEM of tumour volume at 10 weeks after injection is shown. n = 3 mice for each group. e Lysates from Ctrl and OPN KO tumour cells were examined by western blots for P-Stat3, Stat3 and GAPDH. Relative levels of pSTAT3/STAT3 (normalized to Ctrl cells) are shown as mean ± SD on the right. n = 3 independent experiments. f Lysates from Ctrl, Fip200-KD, Atg5 KO and Atg7 KO tumour cells were examined by western blots for P-Stat3, Stat3 and GAPDH. Relative levels of pSTAT3/STAT3 (normalized to Ctrl cells) are shown as mean ± SD on the right (n = 3 independent experiments). g Representative images of immunofluorescent staining for p-STAT3 and DAPI of Ctrl, Fip200 KO, Atg5 KO, Atg7 KO cells and Fip200 KO tumour cells with OPN re-expression (Fip200 KO + OPN), as indicated. Scale bar, 10 μm. Unpaired two-tailed t test was used in c, d, e (right panel), and f (right panel). Source data are provided as a Source Data file.

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