Fig. 1: BOEC functional characterization for VEPs assembly.
From: Directed self-assembly of a xenogeneic vascularized endocrine pancreas for type 1 diabetes
A Immunofluorescence image of BOEC endothelial phenotyping CD31 (green) and Von Willebrand factor (vWF-red), VE-cadherin (green), vascular endothelial growth factor receptor 2 (VEGF-R2-red) and DAPI (blue). Scale bar in µm. Two independent experiments; the representative images were shown; B Flow cytometry BOECs phenotyping for CD31, VE-cadherin, CD34 and KDR (blue and red: histogram for isotype control and positive cells respectively); C WST-1 assay for BOEC proliferation in the presence of standard BOEC culture medium (BCM) or VEP media combination, (n = 8 biological replicates) for 24 or 48 h. Values presented as mean ± SD. BCM vs. VEP media combination 24 h ***p = 0.0002; BCM vs. VEP media combination 48 h ***p = 0.0002; Mann-Whitney U-test D, E BOECs tube formation assay: D Morphology of BOECs tube formation assay in the presence of VEP media, MSM Modified Stabilization Medium, AM Angiogenic Medium, and BCM magnification 4×. Two independent experiments; the representative images are shown. E Analysis of the formed vascular mesh for vascular density analysis in the presence of BCM, AM, MSM and VEP media combination, (n = 10 biological replicates), values presented as mean ± SD (BCM vs. Angiogenic medium *p = 0.0166; BCM vs. VEP media combination *p = 0.0235), one way ANOVA with Dunn’s correction. Source data are provided as a Source Data file.
