Fig. 2: Structure of Cx43-M257 GJICh in GCN conformation.
From: Conformational changes in the human Cx43/GJA1 gap junction channel visualized using cryo-EM
a Cryo-EM map density of Cx43-M257 GJICh in POPE/CHS (condition 7) viewed from the membrane plane (left) and cytoplasmic side (right). The NTHs are colored magenta. Water molecules are shown as red spheres. b Ribbon representation of the structure in (a). c Detailed intramolecular hydrophobic interactions between NTH and TM2. The residues involved in these interactions are drawn as sticks and labeled. d Sequence alignment of A- and C-class connexins. More than 90 and 80% similarly conserved residues are shaded in dark and light gray, respectively. The residues involved in the intramolecular NTH–TM2 interactions are indicated by asterisks. e Detailed intermolecular interactions between NTH and TM2 in a neighboring protomer. NTH and TM2 in the neighboring protomer are colored magenta and gray, respectively.
