Fig. 7: Changes in the pore properties during the GCN-to-PLN conversion of individual protomers.
From: Conformational changes in the human Cx43/GJA1 gap junction channel visualized using cryo-EM
a Constriction diameters of various hemichannel regions with possible arrangements of the PLN protomers. b Comparisons among the solvent-accessible pore radii changing along the pore pathways of GCN, 3:3 mixed, and PLN hemichannel regions are depicted. Pore constriction sites are indicated by asterisks. Extracellular layer (E.C. layer) and intracellular layer (I.C. layer) of pore-occluding lipids are indicated. c Comparisons among GCN, 3:3 mixed, and PLN GJIChs are depicted with pore pathways (top). For clarity, only two protomers facing each other are shown. The NTHs are colored magenta. The π-helix in the middle of TM1 is black. Surface electrostatic potentials of the pore region viewed from the cytoplasmic side (bottom). The surface electrostatic potentials are calculated and colored using Coulombic Surface Coloring in Chimera. The displayed potentials range from −10 (red) to +10 (blue) kT/e. CHS1 and POPE are drawn as sticks. The constriction diameters are labeled. d Ionic currents and selectivity of 3:3 mixed and full PLN GJIChs in the MD simulation. Data are presented as mean ± SE (n = 4). Source data are provided as a Source Data file.
