Fig. 2: DdCBE_Ss achieve high-efficiency editing at multiple mtDNA loci. | Nature Communications

Fig. 2: DdCBE_Ss achieve high-efficiency editing at multiple mtDNA loci.

From: DddA homolog search and engineering expand sequence compatibility of mitochondrial base editing

Fig. 2: DdCBE_Ss achieve high-efficiency editing at multiple mtDNA loci.

a Structural alignment of Ddd_Bc (blue, PDB 6U08) and Ddd_Ss (purple, predicted by ColabFold). Ddd_Bc was split after the red-labeled amino acid G1333 or G1397, Ddd_Ss was split after the orange-labeled amino acid N29 or N94. b Split deaminases fused to left and right TALE proteins targeted to the MT-ATP6 gene. c, d mtDNA editing efficiencies and indel frequencies of HEK293T cells treated with ND5.1-DdCBE (c) or ATP6.1-DdCBE (d). Bc_G1397: Ddd_Bc-G1397 split; Ss_N94: Ddd_Ss-N94 split; Bc_G1333: Ddd_Bc-G1333 split; Ss_N29: Ddd_Ss-N29 split. Shown are mean ± SD; n = 3 independent experiments. The transfection time was 3 days. For c, Ss_N94 and Ss_N29 was compared to Bc_G1397 and Bc_G1333, respectively. P values were calculated by Student’s unpaired two-tailed t-test. Source data are provided as a Source Data file.

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