Fig. 5: E1370N mutation extensively increases the editing efficiency and sequence compatibility of DdCBE_Bc. | Nature Communications

Fig. 5: E1370N mutation extensively increases the editing efficiency and sequence compatibility of DdCBE_Bc.

From: DddA homolog search and engineering expand sequence compatibility of mitochondrial base editing

Fig. 5: E1370N mutation extensively increases the editing efficiency and sequence compatibility of DdCBE_Bc.

a mtDNA editing efficiencies of HEK293T cells treated with ND5.1-DdCBE derived from different Ddd_Bc variants. For Ddd_Bc_L1 and Ddd_Bc_L3, loop 1 and loop 3 sequences in Ddd_Bc are replaced with the corresponding sequence of Ddd_Ss as shown in Supplementary Fig. 11a. Shown are mean ± SD; n = 3 independent experiments. P values were calculated by two-way ANOVA followed by Dunnett’s test. b, c mtDNA editing efficiencies of HEK293T cells treated with ND1-DdCBE (b) and ND5.2-DdCBE (c). Shown are mean ± SD; n = 3 independent experiments. Ddd_Bc_E1370N was compared against Ddd_Bc. P values were calculated by Student’s unpaired two-tailed t-test. Source data are provided as a Source Data file.

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