Fig. 4: Tac1+; CGRP- PBN neurons exert precise temporal control of breathing throughout the maximum physiological range.
From: Parabrachial tachykinin1-expressing neurons involved in state-dependent breathing control
a Example plethysmograph recordings showing entrainment of the respiratory rhythm during 15-s photostimulations of Tac1+; CGRP- neurons at 6, 8, 10, or 12 Hz (a1). Traces are expanded in a2, and overlayed and averaged (bolded lines) in a3 to illustrate relative effects on TI and TE. b Average breathing frequency (replicates shown in light gray) before and during 15-s photostimulations of Tac1+; CGRP- PBN neurons at 6–14 Hz (n = 7 mice). c Cross-correlations of laser pulse and plethysmograph waveforms for data shown in b. Note optimal correlation and entrainment at 11 Hz stimulation frequency. d Probability density histograms of evoked breath frequencies during 6–14 Hz photostimulations relative to the light OFF condition (data pooled from all stimulation frequencies). e Average number of breaths per stimulation for each mouse (n = 7) relative to the stimulation frequency showing a near 1:1 relationship at 8–12 Hz. f Comparison of TI and TE of the evoked respiratory rhythm, and g comparison of maximum cross correlation and corresponding lag time relative to stimulation frequency (n = 7). Means ± SE. Source data and statistical details provided in Source Data file.
