Fig. 2: Thermal adaptation of purified isozymes from seawater samples.
From: Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes
a Optimal temperature (Topt), b denaturation temperature (Td), and e phase transition temperature (Tp) patterns as a function of the MAT at the site from which the 78 esterases originated along the North–South latitudinal transect. c Topt, d Td, and f Tp patterns of 150 esterases from 56 TARA ocean locations (Supplementary Data S4) as a function of the MAT at the site. Topt, Td, and Tp of esterases are determined by measuring the initial hydrolysis rate of 4-nitrophenyl-propionate, the CD ellipticity changes (in millidegrees, mdeg; θ) at 220 nm at different temperatures at a rate of 0.5 °C per min, and performing Constraint Network Analysis (CNA), respectively. Topt and Td values are plotted as mean (n = 3) and related SD are reported in Supplementary Data S3 and S4. Tp values are presented as the mean of five independent MD simulations analysed with CNA and related SEM are given in Supplementary Data S3 and S4. The linear regressions are performed using a two-sided test in R; R2, degrees of freedom, F and p-values are reported in each graph. Significant regressions are reported as blue lines, while non-significant in grey. The blue/grey zone represents the confidence value of 95%. In the case of esterases from the TARA ocean dataset, piecewise regressions were run, and the breakpoints (flexus) where the slope of the regressions significantly changed are indicated with dashed lines on the MAT axis. Red boxes in panels c, d and f indicate the MAT range covered by the Irish Sea–Red Sea transect in panels a, b and e.
