Fig. 3: Ookinete SPMTs contain interrupted luminal helices and are organised in a complex apical pole.
From: Variable microtubule architecture in the malaria parasite
a Tomogram slice through the apical end of an ookinete with SPMTs oriented along the apico-basal axis. A cartoon of an ookinete on the right shows approximate centre positions of lamella in a, b and d. b Slice through an ookinete apex proximal region with SPMTs cut transversally, the annotation colours are the same as in a. SPMTs get closer to the IMC as inner apical collar tapers off (from left to right). The rotational orientation of SPMTs (centre to seam) is indicated with arrows. c Orthogonal sections through EM map determined by SVA of ookinete SPMTs. d Segmentation of an apex proximal region of an ookinete with transversally cut SPMTs, highlighting the two apical collar layers between the SPMTs and IMC. The conoid is shown in green. e Segmentation of the apical pole of an ookinete. The tubulin density of one SPMT was hidden to reveal the ILH. Inset shows a slice through an average volume of the conoid periodic structure. Unannotated slice through tomogram shown in Fig. S3b and full volume in supplemental movie S2.
