Fig. 1: The workflow of the ASGARD drug repurposing pipeline.
From: ASGARD is A Single-cell Guided Pipeline to Aid Repurposing of Drugs
The workflow of the ASGARD pipeline. Diseased and normal cells are paired according to “anchor” genes that are expressed consistently between the two types of cells. The differentially expressed (DE) genes are identified between diseased and normal cells, either within a cluster or within a cell type. Using the consistent DE genes as the input, potential drugs that significantly reverse the pattern of DE genes are identified, using the Kolmogorov–Smirnov (K-S) test with Benjamini–Hochberg (BH) false discovery rate (FDR) adjustment. Next ASGARD estimates and ranks the drug scores for single drugs, by targeting specific cell cluster(s) or all cell clusters.
