Fig. 3: Expression and protease activity of Ssp variants. | Nature Communications

Fig. 3: Expression and protease activity of Ssp variants.

From: Crystal structure of a subtilisin-like autotransporter passenger domain reveals insights into its cytotoxic function

Fig. 3

a Crystal structure of Ssp in cartoon representation displaying location of mutations. b SDS-PAGE analysis of culture supernatant from expression of Ssp variants in E. coli Top10. Data is representative of three independent experiments. c Protease activity of Ssp variants using a fluorescent casein substrate. Error bars represent standard deviation. Ssp variants include wildtype (WT), deletions of Loop 2 (ΔL2), deletion of active site protrusions E2 and E3 (ΔE2, ΔE3, ΔE2/ΔE3), and site-directed mutants of the active site Ser (S314A) and RGD motif (RAE). pBAD denotes vector only control. Mean is plotted with error bars representing the standard deviation of technical replicates (n = 3). Data is representative of three independent experiments.

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