Fig. 2: iLN regulates cold-induced activation of ILC2s, recruitment of eosinophils, polarization of M2 macrophages and beiging of adipocytes in scWAT.

SVF was isolated from inguinal scWAT of 11-week-old iLNX or sham-operated male C57BL/6 N mice after housing at 30 °C for 3 weeks and subsequently subjected to 30 °C or 6 °C for 2 days. a The mRNA expression of type 2 cytokines (n = 5). b The absolute number of ILC2s (Lin^-Cd5^-Cd45⁺Cd127⁺IL33R⁺) in entire scWAT. For Sham_30 °C, iLNX_30 °C and iLNX_6 °C (n = 5) or Sham_6 °C (n = 7). c–e Representative contour plots for ILC2s (left) and quantification of the percentage (right) of IL-5- (c) IL-13- (d) and MetENK- (e) positive cells in activated ILC2s from scWAT (n = 5). f Representative contour plots for eosinophils (CD45⁺CD11b⁺F4/80⁺SiglecF⁺SCC^hi) (left) and quantification of the absolute number of eosinophils in entire scWAT (right) (n = 5). g Representative contour plots from flow cytometry analysis for macrophages (M1, F4/80⁺Cd11b⁺Cd11c⁺; M2, F4/80⁺Cd11b⁺Cd206⁺) (top) and quantifications of the absolute number of M1 and M2 macrophages in entire scWAT (bottom) (n = 5). h Representative histogram overlays of flow cytometric analysis for beige-specific markers (top) and quantification of median fluorescence intensity (MFI) of Cd137 and TMEM26 (bottom) in adipocyte progenitors. For Sham_30 °C (n = 6) or iLNX_30 °C, Sham _6 °C and iLNX_6 °C (n = 5). i Real-time PCR analysis of the mRNA expression of beige progenitor markers (n = 5). All samples are biologically independent replicates. Data are presented as mean ± SEM. All statistical data were assessed using the Mann–Whitney U test. All the p values were two-sided. Source data are available as a Source Data file. See also Fig. S2–S6.