Fig. 4: Replenishment of IL-33 rescues the defective cold-induced beiging in iLN-depleted mice.

a–f Eight-week-old male C57BL/6 N mice after bilateral iLNX or sham operation were housed at 30 °C for three weeks. 250 ng recombinant mouse IL-33 protein (rmIL-33) or PBS (Veh) was directly injected into the scWAT for 4 consecutive days. Mice were then subjected to 6 °C or continued to be housed at 30 °C for 2 days after the first two injections. a Schematic diagram showing the experimental design. b Quantification of the percentage of IL-5-, IL-13- and MetENK-positive cells in activated-ILC2s from scWAT using flow cytometric analysis (n = 5). c, d The mRNA expression of beige-specific markers (c) and thermogenic genes (d) in scWAT (n = 5). e Western blot analysis for UCP1 protein expression in scWAT (top) and densitometric quantification for the relative abundance of UCP1 normalized with HSP90 (bottom) (n = 3). f Representative images of H&E and IHC staining for UCP1 in scWAT. Scale bar, 200 μm. All samples are biologically independent replicates. Data are presented as mean ± SEM. Statistical data were assessed using Mann–Whitney U test (b–d) or unpaired two-tailed Student’s t test (e). All the p values were two-sided. Source data are available as a Source Data file. kDa, relative molecular weight in kilodalton. See also Fig. S12–S13.