Fig. 3: Egr-1 deficiency accelerates liver age-related metabolic dysfunction. | Nature Communications

Fig. 3: Egr-1 deficiency accelerates liver age-related metabolic dysfunction.

From: The rhythmic coupling of Egr-1 and Cidea regulates age-related metabolic dysfunction in the liver of male mice

Fig. 3: Egr-1 deficiency accelerates liver age-related metabolic dysfunction.The alternative text for this image may have been generated using AI.

A Liver TG levels of WT and Egr-1-LKO mice at 2 months, 6 months, 12 months, and 21 months (WT: 2 months: n = 7; 6 months: n = 7; 12 months: n = 8; 21 months: n = 6; Egr-1 LKO: 2 months: n = 6; 6 months: n = 5; 12 months: n = 6; 21 months: n = 6 biologically independent animals). B, C H&E staining and Oil Red O staining of WT and Egr-1-LKO mice at 2 months, 6 months, and 21 months of age. D, E Liver and serum-free fatty acid levels of WT and Egr-1-LKO mice at 6 months (n = 6 or 7 biologically independent animals in WT group and n = 5 or 6 biologically independent animals in Egr-1 LKO group). F Sirius Red staining of 21-month-old WT and Egr-1-LKO mice. G mRNA levels of the liver fibrosis marker a-SMA (n = 9 biologically independent animals). H β-Galactosidase staining indicates the aging process. I Survival curves of WT and Egr-1-LKO mice. Each experiment was repeated three times independently (WT: n = 22; Egr-1 LKO: n = 23 biologically independent animals). Data were represented as mean ± SEM. Exact p values are depicted in the figure. Statistical analysis was performed using one-way ANOVA for A and unpaired two-tailed Student’s t-test for D, E, and G. Scale bar: 100 µm. Source data are provided as a Source Data file.

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