Fig. 5: Additional analysis of cell injury compounds in cell painting.

Cellular injury compounds were tested at 24- and 48-h to determine the effect of compound treatment times on cell-painting (CP) readouts. a CP phenotypes after 24- and 48-h compound treatments are correlated. b Replicates of active CP compounds are correlated at 24- and 48-h compound treatment times. The replicate correlation is defined by the average correlation between each replicate pair (6 comparisons total). There are generally strong correlations between compound treatments with strong signals (red). c CP clusters from 24- and 48-h compound treatments are correlated. The horizontal- and vertical-axis correspond to the correlation of the 9 clusters to the 24- and 48-h treatment profiles, respectively. d CP profiles from 24- and 48-h compound treatments are correlated. Tanglegram shows connections between compound treatments from 24- and 48-h treatment dendrograms. e Cellular health biomarkers (AUC) are grossly correlated at 24- and 48-h treatment times. Compounds that decrease confluence, active caspase 3/7, and decrease membrane integrity at 24 h generally produce similar changes at 48 h. N.B., while many points lie along the parity line, some have larger values at 48 h. f Select CP profiles comparing 24- and 48-h compound treatments. Note the trends of relative cell number and CP activity scores are similar at both time points. Data are mean ± SD of four intra-run technical replicates each performed on separate microplates. Source data are provided as a Source Data file.