Fig. 6: Microglial AR is required for DHT-induced control of microglia response to demyelination. | Nature Communications

Fig. 6: Microglial AR is required for DHT-induced control of microglia response to demyelination.

From: Androgens show sex-dependent differences in myelination in immune and non-immune murine models of CNS demyelination

Fig. 6

a Scheme of the experimental paradigm. b–g Visualization and quantification of Arg-1 expression in Iba1-expressing microglial cells in b, c, OPC differentiation in d, e and GFAP immunostaining of astrocytes in f, g at 7 days after stereotaxic injection of LPC into the corpus callosum of ovariectomized female mice expressing (AR wt/wt) or not (AR fl/fl) AR in microglia and treated with the drug vehicle (Veh) or DHT. The dashed lines delineate the lesions. Scale bars: 50 µm. Data are presented as mean values ± SEM from n = 6 mice/condition examined in two independent experiments (3 slices/per animal). P values (c, e, g,) were calculated by using the two-way ANOVA test together with Tukey’s multiple comparisons test. **p = 0.0069 (e left), p = 0.0061 (e right); ****p < 0.0001 c, p = 0.0001 g; n.s. not significant. Source data are provided as a Source Data file.

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