Fig. 8: Effect of Spi2a overexpression in cytokine storm and heart dysfunction in animal sepsis models. | Nature Communications

Fig. 8: Effect of Spi2a overexpression in cytokine storm and heart dysfunction in animal sepsis models.

From: N6-methyladenosine of Spi2a attenuates inflammation and sepsis-associated myocardial dysfunction in mice

Fig. 8

Macrophage-depleted wild-type mice were reconstituted with wild-type BMDMs transduced with control- or Spi2a-lentivirus prior to LPS challenge. a Kaplan–Meier survival curves. n = 10 in each group. b Real-time PCR for cytokine expression in peritoneal macrophages. n = 5 in each group. c ELISA for TNF, IL-6, IL-1β, IFNγ, HMGB1, IL-1α and IL-33 in serum. n = 5 in each group. d Real-time PCR for cytokine expression in heart tissue samples. n = 5 in each group. e TUNEL staining for dead cells (indicated by white arrows) detection in heart tissues (top) and quantitative analysis of TUNEL staining-positive cells in heart tissues displayed as box-and-whiskers plots (bottom). n = 20 fields of view in each group. f Macrophage-depleted wild-type mice were reconstituted with M14f/f or M14−/− BMDMs transduced with control- or Spi2a-lentivirus prior to LPS challenge, then survival experiments were performed, n = 10 in each group. Ctrl, Control; mM14−/−, M14−/− macrophages. Data are shown as mean ± SD. For box-and-whisker plots, the bottom line shows the lower quartile, top line the upper quartile, and whiskers the maxima/minima. Log-rank tests (a, f) and two-way two-sided ANOVA (b–e) were used for statistical analyses.

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