Fig. 3: Showcasing DNA cytoskeletal complexity and biophysical control inside protocells. | Nature Communications

Fig. 3: Showcasing DNA cytoskeletal complexity and biophysical control inside protocells.

From: Creating complex protocells and prototissues using simple DNA building blocks

Fig. 3

A Two fiber types compartmentalized inside GUVs, schematic representation (left), and corresponding CLSM images (right), fiber 1 (magenta) and fiber 2 (green), scale bar 2 µm. B Controlling location of cytoskeletal DNA fibers using cholesterol lipid anchors, schematic representation, cholesterol modifications (orange spheres) and corresponding CLSM images, cholesterol fibers (magenta) and control unmodified fibers (green), scale bar 2 µm. C Controlling DNA fiber alignment in real-time using magnetism, DNA fibers coated in magnetic beads (black spheres) which align with an external magnetic field inside a GUV (grey channel), arrow direction denotes the direction of the external magnetic, scale bar 2 µm. D Immobilization of large unilamellar vesicles (LUVs) (magenta channel) along cytoskeleton DNA fibers (green channel) inside protocells, scale bar 2 µm.

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