Fig. 3: Tracking simulation in an E. coli-like geometry.
From: Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization
Example trajectory with HMM analysis of 100 simulated trajectories. a Upper left; example trajectory with x (red) y (green) z (blue) estimated position coordinates with corresponding photon counts (bottom). Upper right; the deviation away from ground truth with the SD of the distribution in the legend, (bottom) is the photon count distribution. b Left; ECVE diffusion point estimation (gray) along the trajectory in (a), ground truth diffusion is in green, and the HMM prediction in red. Right; the distribution of estimated diffusions over the trajectory in (a). c Distribution of all ECVE values from 100 trajectories. The 3 state HMM model prediction of the emission distributions is plotted on top of the histogram, and the inverse-gamma mean and SD of each emission distribution is reported with standard error from a bootstrap analysis over trajectories in the legend. Inset, 3D representation of the cell volume with ground truth trajectory in black and the estimated path in yellow. Source data are provided as a Source Data file.
