Fig. 5: Schematic drawing of the transport process and conformational switching of OxlT.

The conformational landscape of OxlT along the periplasmic and cytoplasmic gate distances is shown in the top right panel. The Cα distances of the gate residues in MD simulations of the occluded and outward-open states and Gln34-induced transition are shown in red, yellow and blue, respectively. The gate-residue distances in the current occluded (PDB ID 8HPK) and outward-facing (PDB ID 8HPJ) crystal structures as well as the Nark inward-facing crystal structure (PDB ID 4U4T) are also shown.