Fig. 6: Loss of FYN abolishes the cardioprotective effects of FGF18 in vivo.
From: Fibroblast growth factor 18 alleviates stress-induced pathological cardiac hypertrophy in male mice
a, b The mice were injected with AAV9-LacZ or AAV9-cTnT-FGF18 intravenously before Sham or TAC operation. a Western blotting with p-FYN (IP: FYN, IB: p-Src) and FYN antibody. Quantification of relative FYN activity and protein expression levels (right panel). n = 5. Data represent means ± SD. one-way ANOVA with Tukey multiple comparisons test. b qRT-PCR analysis of FYN levels. n = 5. Data represent means ± SD. one-way ANOVA with Tukey multiple comparisons test: n.s. = not significant. c–i AAV9-cTnT-FGF18 and control vector were injected intravenously into tail veins of 6 weeks old male C57BL/6J mice, respectively. One week after the injection, these mice were intravenously injected with the adeno-associated virus (AAV9-cTnT-sh-FYN; AAV9-Scramble) into tail veins. One week after the injection, these mice were subjected to Sham or TAC operation for 6 weeks. c Histological analyses of the HE staining and WGA staining (n = 5. Scale bar = 0.6 mm for upper HE staining; scale bar = 20 μm for lower WGA staining). Statistical results for the sectional cell area (right panel). n = 5. d Real-time quantitative PCR assays. n = 5. e Statistical results for the ratios of HW/BW in the indicated groups. n = 5. f Echocardiographic data for LVEF and LVFS are shown. n = 5. g PSR staining and Quantification (right panel). n = 5. Scale bar = 450 μm, and then zoom in 5 times. h Left ventricular collagen quantification by hydroxyproline assay (μg/mg). n = 5. i Levels of the oxidative damage marker 3-NT. The quantitative analysis of protein immunoblots (right panel), n = 5. j Total ROS levels (by DCFH-DA probe) were quantified. n = 4. k Hydrogen peroxide levels (by Amplex Red assay) quantified in different groups. n = 5. One-way ANOVA was followed by a post hoc Fisher’s comparison test. l Quantification of relative protein levels. n = 5. c–i All quantitative data are reported as means ± SD, one-way ANOVA with Tukey multiple comparisons test. All numbers (n) are biologically independent animals. Source data are provided as a Source data file.
