Fig. 3: TaWD40-4B.1C has a stronger interaction capacity with canonical catalases than TaWD40-4B.1T. | Nature Communications

Fig. 3: TaWD40-4B.1C has a stronger interaction capacity with canonical catalases than TaWD40-4B.1T.

From: Allelic variation of TaWD40-4B.1 contributes to drought tolerance by modulating catalase activity in wheat

Fig. 3: TaWD40-4B.1C has a stronger interaction capacity with canonical catalases than TaWD40-4B.1T.The alternative text for this image may have been generated using AI.

a The yeast two hybridization assay reveals that both TaWD40-4B.1C and TaWD40-4B.1T interact with canonical TaCAT1s and TaCAT3s but not noncanonical TaCAT2s. b The bimolecular fluorescence complementation assay confirms the interaction between TaWD40-4B.1C/TaWD40-4B.1T and TaCAT3A. DsRed-SKL is the DsRed fused with peroxisome targeting sequence SKL serving as the peroxisome marker. Bar = 5 μm. c TaCAT1s/TaCAT3s and TaCAT2s are canonical and non-canonical catalases, respectively. d The split-luciferase complementation imaging showing TaWD40-4B.1C has a stronger interaction capacity with TaCAT1A and TaCAT3A than TaWD40-4B.1T. e, f Co-immunoprecipitation (e) and pull-down (f) assays indicate that TaWD40-4B.1C has a stronger interaction capacity with TaCAT3A than TaWD40-4B.1T. Source data are provided as a Source data file.

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