Fig. 2: Validation of the Well-TEMP-seq performance. | Nature Communications

Fig. 2: Validation of the Well-TEMP-seq performance.

From: Well-TEMP-seq as a microwell-based strategy for massively parallel profiling of single-cell temporal RNA dynamics

Fig. 2

a Bar plot of nucleotide substitution rates in K562 cells (K562), 4sU-labeled K562 cells (K562_4sU), IAA-treated K562 cells (K562_IAA), and 4sU-labeled and IAA-treated K562 cells (K562_4sU_IAA). b Box plot of the fraction of labeled transcripts per cell in 4sU-labeled K562 cells and 4sU-labeled and IAA-treated K562 cells. n = 2000 cells for each group. Boxplots include centerline, median; box limits, upper and lower quartiles; and whiskers are highest and lowest values no >1.5× interquartile range. c Single-cell/barcoded bead pairing rate in Well-TEMP-seq and scNT-seq. The pairing rate in scNT-seq was calculated by Poisson distribution. d Cell recovery rate after IAA chemical treatment in Well-TEMP-seq and sci-fate. e Violin plots showing the fraction of T-C mismatch UMIs per cell of different genes in 4sU-labeled K562 cells and 4sU-labeled and IAA-treated K562 cells. Left, high turnover gene (MYC); middle, median turnover gene (PDLIM5); right, low turnover gene (GAPDH). Source data are provided as a Source Data file.

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