Fig. 3: Ai and LpWF occupy separate niches within the proventriculus.
A Strain interactions influence colonization efficiency, as seen by dose-response curves for Ai fed to germ-free flies (open green circles), Ai-pre-colonized flies (filled yellow circles), or Lp-pre-colonized flies (black-filled green squares). Z-test of differences in proportion versus Ai into germ-free flies: dose 102.3 CFUs/fly, p = 8.1×10−4; dose 103.7 CFUs/fly: p = 4.8 × 10−9; dose 105 CFUs/fly: p = 8.7 × 10−6. n ≥ 12 flies/data point. Error bars represent 1 standard error of the proportion. B Ai abundance at 5 dpi does not differ between flies mono-colonized with Ai versus pre-colonized with LpWF then fed Ai. n ≥ 65 flies/treatment; two-tailed unpaired t-test, p = 0.38; ns indicates not significant. C LpWF abundance 5 dpi does not differ between flies mono-colonized with LpWF versus pre-colonized with Ai then fed LpWF. n ≥ 53 flies/treatment; two-tailed unpaired t-test; p = 0.06; ns indicates not significant. B, C: Center of box is median; box encloses 25th to 75th percentiles; whiskers indicate minimum and maximum. D Confocal microscopy of Lp and Ai co-colonization. Ai (green) and LpWF (red) occupied the same regions of the foregut 1 dpi. Scale bar: 100 µm. E, F x,z-section of Ai and LpWF sectors. G TEM cross-section of Ai and LpWF co-colonizing the anterior proventriculus. Scale bar: 5 µm. H Detail of (G) with LpWF and Ai cells pseudocolored. Scale bar: 2 µm. Source data are provided as a Source Data file.
