Fig. 2: Insulation of TAD and enhancer-promoter looping were reduced by CTCF depletion in BMDCs. | Nature Communications

Fig. 2: Insulation of TAD and enhancer-promoter looping were reduced by CTCF depletion in BMDCs.

From: CTCF controls three-dimensional enhancer network underlying the inflammatory response of bone marrow-derived dendritic cells

Fig. 2

a Either LPS stimulation or CTCF depletion does not significantly affect higher-order segregation of A and B compartments when evaluated by cis-Eigenvector 1 values. b–d CTCF is required for proper insulation of TAD in BMDCs. Number of boundaries (b), distance between boundaries (c), and boundary strength (d) obtained with Hi-C data. Box-plot with midline = median, box limits = Q1 (25th percentile)/Q3 (75th percentile), whiskers = minimum and maximum values. Significance was calculated using a one-way ANOVA with Tukey’s multiple comparisons. The sample sizes (n) were labeled in the figure. e, f Depletion of CTCF disrupts enhancer-promoter looping in BMDCs. Number of loops (e) and distribution of regulatory elements at the anchors of the high-confidence H3K27ac HiChIP loops (f). Source data are provided as a Source Data file.

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