Fig. 3: Localized distribution of ipRGCs capture ground luminance.
a–f Confocal micrographs and density maps of neurons labeled in GlyT2Cre mice. a Melanopsin-positive ipRGCs in the ventral retina are negative for EGFP. b In the dorsal retina, many ipRGCs are EGFP-positive and overlap with EGFP-negative ipRGCs. c S-cone opsin staining illustrates sparse labeling in the dorsal retina and dense staining in the ventral retina. d Location map of melanopsin immuno-positive ipRGCs (n = 3314). Dorsal-ventral density is plotted on the right (100 μm bins across the Y axis). Square = region shown in a. D dorsal, V ventral, N nasal, T temporal. e 293 ipRGCs were EGFP-positive and restricted to a region of the retina that is low in (f) S-cone opsin density. Squares = regions in b and c above. g Illustration of the GlyT2Cre ipRGCs in the dorsal retina of the mouse encoding light in the ventral visual field compared to all ipRGCs. S-opsin-containing cones encode the upper visual field. Mouse printed with permission from © 2023 The Jackson Laboratory. h Distribution of GlyT2Cre ipRGCs in both eyes plotted in a sinusoidal projection adapted from Bleckert et al. 2014. o.n. optic nerve. i Confocal micrograph of melanopsin, TdTomato, and RBPMS co-staining in the dorsal GlyT2Cre;Ai9 mouse retina. The left arrows indicate RBPMS-positive GlyT2Cre ipRGCs. All melanopsin-negative, TdTomato-positive cells are RBPMS negative (arrowheads). j Quantification of total melanopsin-positive cell bodies (n = 3 retina from n = 3 animals), cell bodies co-labeled with TdTomato & RBPMS (n = 4 retina from n = 4 animals), and cell bodies co-labeled with TdTomato and melanopsin (n = 9 retina from n = 6 mice; ****p < 0.0001; ns p = 0.9981). k All GlyT2Cre-positive ipRGCs are melanopsin-positive. Percentage of TdTomato-positive and RBPMS-positive cell bodies that are melanopsin-positive (left) compared to melanopsin-negative (right) identified from whole-mount GlyT2Cre;Ai9 retinas (n = 4 retina). Data were presented as mean values ± SEM. Statistical significance was assessed using one-way ANOVA using Å Ãdák’s multiple comparisons test. Scale bars in a = 20 μm, b = 20 μm, c = 25 μm, d–f = 0.5 mm, i = 50 μm. Source data are provided as a source data file.
