Fig. 3: NR2F2 isoform 2 promotes melanoma metastasis.
a–d 4L (N = 9 shSCR, N = 8 shA athymic/nude mice) and e–h 12-273BM ( N= 9 shSCR, N = 12 shA, N = 11 shB NSG mice) cells labeled with lentiviruses that constitutively express luciferase and mCherry, and transduced with lentiviruses expressing shSCR or shNR2F2-Iso2 (shA, shB) along with green fluorescent protein (GFP), were instilled into mouse hearts by ultrasound-guided injection. a, e Bioluminescence and fluorescence images of mice and their corresponding organs (brains, lungs and ovaries in a; livers in e) ex vivo at the endpoint of one representative experiment. b, f In Vivo Imaging System (IVIS) measurements showing significant differences in radiance levels between groups during tumor progression (two-way ANOVA, bars represent SEM). c Bar graphs showing that the average number of lung metastases per tissue section is significantly reduced in mice injected with shNR2F2-Iso2 compared to those injected with shSCR expressing 4L cells (two-tailed unpaired T-test, bars represent SEM). d Representative H&E-stained tissue sections. Circles identify metastases. Scale bar = 100 μm. Bar graphs showing g average number of metastases per liver section and h GFP intensity of livers indicate significantly reduced metastatic burden in mice injected with shNR2F2-Iso2 compared to those instilled with shSCR expressing 12-273 BM cells (two-way ANOVA, bars represent SD). Error bars represent standard error of the mean. Source data are provided as a Source Data file.
