Fig. 5: RECQ4 N-terminal interaction with APC/C promotes replisome assembly.

a (left) A schematic diagram of RECQ4 wild type (WT), Q757X, and Q757X-internal-deletion (ID) double mutant. (right) Fold changes in the indicated co-purified proteins normalized to the bait FLAG-RECQ4 proteins relative compared to the FLAG-RECQ4 WT immunoprecipitation (IP) based on the western blot analysis shown in (f). b–d Quantification of DNA binding efficiency of recombinant His-tagged, FLAG-tagged RECQ4 Q757X (b), ID (c), or Q757X-ID (d) compared to WT RECQ4. e Representative western blot analysis of the indicated proteins in whole-cell extracts (WCE) prepared from RECQ4 knockdown (KD) HEK293 cells stably expressing RECQ4 WT, Q757X, or Q757X-ID. Actin was used as a loading control. f Representative western blot analysis of the indicated proteins co-immunopurified with FLAG-RECQ4 WT, Q757X, or Q757X-ID proteins prepared from WCE shown in (e). g Representative western blot analysis of the indicated proteins in chromatin-bound (CB) fractions prepared from RECQ4 WT, Q757X, or Q757X-ID expressing cells. LAMIN A/C was used as a loading control. h Representative real-time cell growth assays to measure cell growth rate of RECQ4 KD cells with or without stable expression of FLAG-RECQ4 WT, Q757X, or Q757X-ID mutant. Source data are provided as a Source data file. n = 3 (biologically independent samples). Data are presented as mean values ± SD.