Fig. 4: Origin and evolution of plant Δ5-desaturases. | Nature Communications

Fig. 4: Origin and evolution of plant Δ5-desaturases.

From: The Torreya grandis genome illuminates the origin and evolution of gymnosperm-specific sciadonic acid biosynthesis

Fig. 4: Origin and evolution of plant Δ5-desaturases.

a Maximum likelihood phylogeny of plant desaturases. TgDES1 is clustered within a group (clade 1) close to a sister clade (clade 2) comprising Δ6- and Δ8- desaturases. b Structure modeling of TgDES1 and the desaturases from Arabidopsis (AtSLD2) and Anemone leveillei (AL21). Protein structures were modeled with AlphaFold2 and the bioactive center of each protein comprising three histidine-rich motifs is marked with yellow. c Comparison of conserved motifs in different desaturase groups showing in a. d Detection of SCA and its precursor in N. benthamiana leaves expressing Arabidopsis AtSLD2 with conserved histidine-rich motifs (motif2 and motif3) replaced by those from TgDES1 of T. grandis. AtSLD2, AtSLD2-Motif2, and AtSLD2-Motif3 are lines harboring Arabidopsis wild-type AtSLD2 gene, AtSLD2 with motif2 from TgDES1, and AtSLD2 with motif3 from TgDES1, respectively.

Back to article page