Fig. 2: Male-derived pheromones attract mated females to oviposition sites.

A Overview of the main sex-specific pheromones transferred by males (blue) and females (red) during mating. B Oviposition preference to pheromone extracts of oenocyte ablated (Oe^-), w¹¹¹⁸, or mated flies of the indicated genotypes (see Fig. S1A–B for controls). C Oviposition preference to two, six and twelve times the single fly pheromonal equivalent of cis−11-vaccenyl acetate (cVA: 560–1680–3360 ng), 7-tricosene (7-T: 280–840–1680 ng), 9-tricosene (9-T: 28–84–168 ng; see Fig. S1C) and 7,11-heptacosadiene (7,11-HD: 620–1860–3360 ng) and combinations thereof. Asterisks above a treatment indicates attraction differing significantly from zero (0.0 dashed line—indicating no preference) as determined by two-tailed Wilcoxon signed rank tests (n = 20 for all treatments). Differences in preference across treatments was tested with a GLM with quasibinomial error distribution and is indicated above the bar. *P < 0.05; **P < 0.01; ***P < 0.001. No asterisks means no significant difference from zero for the Wilcoxon signed rank tests. The center line of the box plots denotes the median value (50th percentile), the box contains the 25th to 75th percentiles of dataset. The black whiskers mark 1.5 times the interquartile range. See Fig. S1D for solvent control treatments and Tables S4–S7 for the full outcome of the statistical analyses. Source data are provided with this paper.