Fig. 9: Fetal intestinal samples harbor cells with proliferative capacity in vitro.

a Biaxial plots showing the gating strategy to identify immune subsets in a human fetal intestinal sample from gestational week 16 cultured in medium alone or medium with IL-7 for 4 days. The colored gates indicate the identified immune subsets. Data represent four independent experiments. b Histogram showing CellTrace™ Violet dye dilution in the indicated immune subsets from the human fetal intestine. Dashed lines represent the fetal intestinal sample cultured in medium, while solid lines represents the fetal intestinal sample cultured in medium with IL-7. c Histogram showing CellTrace™ Violet dye dilution by the indicated immune subsets from a PBMCs sample. Dashed lines represent the PBMCs cultured in medium, while solid lines represents the PBMCs cultured in medium with IL-7. d CTV^diluted cells of the immune subset indicated from human fetal intestinal samples in both culture conditions. Data were from four independent experiments (n = 4). Error bars indicate mean ± s.e.m. Wilcoxon signed-rank test with one-tailed for comparisons. e Comparison of the mean MFI values of CD40L expression between culture in medium alone and in the presence of IL-7. Data were from four independent experiments (n = 6). f Comparison of the percentages of CD40L⁺granzyme B⁺ expressing cells within CD3^–CD7⁺ ILCs, CD4^–CD8^– T cells, CD4⁺ T cells and CD8⁺ T cells in both culture conditions. Data were from four independent experiments (n = 6). Error bars indicate mean ± s.e.m. Wilcoxon signed-rank test with one-tailed for comparisons.