Fig. 4: Nucleotide binding halts CMG diffusion.
From: Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation
a Position vs. time plots of CMG spots in the presence of ATPγS; CPA fits are plotted in black, static traces are shown in light gray and mobile traces are shown in all other colors. b Fraction of static CMG spots in the presence of ATPγS (Nspots = 34); the results from Fig. 2d, are shown as light bars for comparison; error bars show the standard error of proportion. c Model proposed to explain experimental motion results; c-i proposed two populations of CMG present in the ensemble CMG activation reaction; c-ii summary of experimental outcomes in Figs. 2 and 4a, and proposed explanation of their origins. d Fluorescent scan of an SDS-PAGE gel showing the amount of Cdc45LD555 left on linear DNA bound to magnetic beads at one end and containing either a free end or an end capped with a covalently crosslinked methyltransferase. e Densitometry quantification of the experiment shown in d showing the average normalized intensity of three replicates together with their standard deviation. Data points are connected by solid lines to guide the eye. Source data are provided as a Source Data file.
