Fig. 2: Presynaptic UNC-43/CaMKII is required for the GABAergic synaptic localization of GABAARs.
a, b Schematic illustration of labeling UNC-57 at the GABAergic motor neurons by split GFP complementary system. Seven copies of the split GFP11 were inserted into the C-terminal of unc-57 genomic loci by CRISPR-Cas9 system. The split GFP1-10 was expressed in GABAergic motor neurons by unc-25 promoters. c, d The colocalization coefficient between GABAARs and GABAergic synaptic boutons was decreased in the unc-43 mutants; this was rescued by transgenic expression of UNC-43 in GABAergic motor neurons (G. rescue). Pearson’s correlation coefficients between the intensities of GABAergic bouton marker UNC-57 (green, labeled by UNC-57-split GFP under unc-25 promoter) and postsynaptic GABAARs (magenta) were used to assess the localization of GABAARs at inhibitory synapses. Representative images (c-top, scale bar, 10 μm), corresponding line scan curve (c-bottom), and mean Pearson’s correlation coefficients +/− SEM (d, Ns represent the number of animals tested) are shown. Kruskal–Wallis test with post-hoc Dunn’s test. ***p < 0.001. Source data are provided as a Source Data file.
