Fig. 5: UNC-43/CaMKII’s recruitment of GABAARs requires both MADD-4B and NRX-1α. | Nature Communications

Fig. 5: UNC-43/CaMKII’s recruitment of GABAARs requires both MADD-4B and NRX-1α.

From: UNC-43/CaMKII-triggered anterograde signals recruit GABAARs to mediate inhibitory synaptic transmission and plasticity at C. elegans NMJs

Fig. 5: UNC-43/CaMKII’s recruitment of GABAARs requires both MADD-4B and NRX-1α.The alternative text for this image may have been generated using AI.

a UNC-43 functions in the same pathway with MADD-4B and NRX-1α to stabilize postsynaptic NLG-1 localization. NLG-1-GFP fluorescence intensities in the body-wall muscles in wild type and mutants were shown. Representative images (left, scale bar 10 μm, Pseudo-color: Fire) and mean puncta intensities +/− SEM (right, Ns represent the number of animals tested) are shown. The data for WT and unc-43 are the same as in Fig. 4c. Kruskal–Wallis test with two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli correction for multiple comparisons. The scatter plotted data labeled with different letters are significantly different (p < 0.05). b, c Both MADD-4B and NRX-1α are required for UNC-43’s recruitment of GABAARs. TagRFP-UNC-49 fluorescence in dorsal nerve cords in wild type and mutants is shown. Representative images (left, scale bar 10 μm, Pseudo-color: Red Hot) and mean puncta intensities +/− SEM (right, Ns represent the number of animals tested) are shown. In b, Kruskal–Wallis test with two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli correction for multiple comparisons. The scatter plotted data labeled with different letters are significantly different (p < 0.05). In c, Kruskal–Wallis test with post-hoc Dunn’s test. **p < 0.01, ***p < 0.001, n.s. not significant. For a–c, source data are provided as a Source Data file.

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