Fig. 9: UNC-43/CaMKII is required for activity-dependent plasticity at GABAergic synapses. | Nature Communications

Fig. 9: UNC-43/CaMKII is required for activity-dependent plasticity at GABAergic synapses.

From: UNC-43/CaMKII-triggered anterograde signals recruit GABAARs to mediate inhibitory synaptic transmission and plasticity at C. elegans NMJs

Fig. 9: UNC-43/CaMKII is required for activity-dependent plasticity at GABAergic synapses.The alternative text for this image may have been generated using AI.

a Presynaptic UNC-43 is required for activity-dependent GABAergic plasticity. TagRFP-UNC-49 puncta fluorescence intensity was increased after GABAergic motor neuron excitation by ChIEF activation in wild type and in the unc-43 gain-of-function mutants, but not in the unc-43 loss of function mutants, and this was rescued by transgenic expression of UNC-43 in GABAergic motor neurons (G. rescue). Representative images (left, scale bar 10 μm, Pseudo-color: Red Hot) and mean puncta intensities +/− SEM (right, Ns represent the number of animals tested) are shown. Kruskal–Wallis test with two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli correction for multiple comparisons. *p < 0.05, n.s. not significant. b Schematic illustration of split GFP experimental design to label endogenous UNC-43 at GABAergic motor neurons. Seven copies of the split GFP11 were inserted into the N-terminal of unc-43 genomic loci by the CRISPR-Cas9 system. The split GFP1-10 was expressed in GABAergic motor neurons by unc-25 promoters. c The GABAergic presynaptic localization of UNC-43 was increased after GABAergic motor neuron excitation by ChIEF activation. split GFP labels endogenous UNC-43 in GABAergic neurons. Representative images (left, scale bar 10 μm, Pseudo-color: Fire) and mean puncta intensities +/− SEM (right, Ns represent the number of animals tested) are shown. Two-tailed Mann–Whitney test. *p < 0.05. d MADD-4B, NRX-1α, and NLG-1, but not FRM-3, are required for activity-dependent plasticity at GABAergic synapses. Representative images (left, scale bar 10 μm, Pseudo-color: Red Hot) and mean puncta intensities +/− SEM (right, Ns represent the number of animals tested) are shown. The data for WT is the same as in a. Kruskal–Wallis test with two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli correction for multiple comparisons. *p < 0.05, **p < 0.01, n.s. not significant.For a, c, d, source data are provided as a Source Data file.

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